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Fluorescent labeled Helicobacter pylori motility
Species name:Helicobacter pylori
Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio, United States of America Professor Marshall H. Montrose
The motility of wild-type H. pylori Strain SS1 was observed by tracking fluorescently labeled cells using a fast-scan confocal microscope. The cells swam in straight or arced lines at mean velocity of 25.5±0.7 µm/sec, with stopping at the frequency of 0.13±0.05 per s, and slowed at pH 5. Fluorescently labeled H. pylori was loaded into an uncoated μ-Slide chamber (ibidi) and monitored on the inverted fast scan confocal microscope (Zeiss LSM 7 LIVE), with a scan speed at 100 ms/frame.