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Video archives
Here, we establish an online video library where a series of movies relevant to motility are available. The miscellaneous category includes bacteria, eukaryotes, and archaea, viruses, proteins, and synthetic polymers. The movies that are meaningful in the biology field will be uploaded in both Japanese and English.
For the contributors who plan to upload your video, you should keep in mind the following suggestions:
(1) the video which is relative to the object of your research
(2) the video about microbe found in the research activity of the super-science high school or biological clubs are encouraged to upload
(3) Do not forget to add the link of your video which has been published (Please make sure the copyright)
(4) If you think some videos in the old textbook are valuable to upload, please let us know.
Video List
Eukaryote
Lecane sp.
Species name:Lecane sp.
Bureau Swerage, Tokyo Metropolitan Government
The size of Lecane is 150-160 μm in length. The body is flat and ovoided. It has two tails and can draw the tail into the lorica. Lecane move around the flock with the tail. The food sourceo are bacteria and protozoa.
Eukaryote
A skilful navigator
Species name:Loxodes or Remanella
AL-Museum AL-Museum
In spite of having a long body, this ciliate is flat like a ribbon. The organism is thus able to move delicately through the narrow spaces between particles.
Eukaryote
Litonotus sp.
Species name:Litonotus sp.
Bureau Swerage, Tokyo Metropolitan Government
The size of Litonotus is 70-170 μm in length. Some specie is about 500 μm. They are rounded with short cilia. Long cilia crowds around the mouth. They glide back and forth. The shape of body do not deform when they move.
Eukaryote
Ciliate with a very long neck
Species name:Litonotus
AL-Museum AL-Museum
This ciliate has a very long and narrow neck with a mouth at the bent tip. Twisting its body allows it to move through the narrow openings of aggregates. When it changes directions, its thin body flutters like a ribbon in the wind.
Prokaryote
Part 1: Bacterial pathogenesis: the Listeria paradigm
Species name:Listeria
Pasteur Institute Pascale Cossart
Cossart begins her talk with an overview of microbiology and then focuses on the bacterium Listeria monocytogenes, a food-borne, intracellular pathogen. Cossart explains how Listeria enter epithelial cells, move around inside cells, and spread between cells. She describes how her lab identifed Listeria virulence genes by comparing the sequence of a non-pathogenic species of Listeria with the sequence of L. monocytogenes. They found that Listeria uses a wide variety of strategies to infect and proliferate within its host.
Prokaryote
Part 2: Exploring "New Microbiology" with Listeria monocytogenes
Species name:Listeria
Pasteur Institute Pascale Cossart
In Part 2, Cossart describes how modern molecular and cell biology techniques have yielded new concepts in microbiology. For example, small non-coding RNAs that are differentially expressed and necessary for virulence have been identified. Short and long antisense RNAs with multiple regulatory functions also have been found. It seems likely that these, and other newly identified mechanisms of bacterial regulation, may prove common to different bacterial species.
Prokaryote
Part 3: Cell biology and infection: lessons from Listeria
Species name:Listeria
Pasteur Institute Pascale Cossart
In her final talk, Cossart reviews the many cellular processes impacted during infection with Listeria. She successively discusses activation of clathrin-mediated endocytosis during bacterial entry, nucleation of actin comet-tails by bacterial ActA, post-translational modifications during infection (in particular deSUMOylation), mitochondrial targeting, and finally chromatin remodeling and epigenetic regulation during infection. Listeria is a true cell biologist, manipulating all aspects of cell function.
Eukaryote
A Gliding Gastrotrich
Species name:Lepidodermella sp.
Gakushuin University Jun Kashiwazaki
Gastrotrichs glide on a solid surface by motility of ventral locomotor cilia. Gastrotrichs were collected from Chiarai pond in Gakushuin University. DIC, real time (30 fps)
Prokaryote
Actin Filament Interacting with Listeria monocytogenes
Species name:Listeria monocytogenes
Center for Cell Dynamics, University of Washington, Friday Harbor, Washington, United States of America Professor Jonathan B. Alberts
Listeria monocytogenes moves by actin-based motility. A close-up look at the interaction of a single polymerizing filament with the bacterium based on in silico reconstitution. This filament has an artificially durable link with an ActA protein on the bacterium's surface; these links are typically very transient. The tip-clearance (drawn with a cyan line), the polymerization probability, the capping probability, and the Arp2/3 binding probability are reported at each simulation time-step
Molecule and Protein
Motion initiation and persistence of actin-based motility
Species name:Listeria monocytogenes
Center for Cell Dynamics, University of Washington, Friday Harbor, Washington, United States of America Professor Jonathan B. Alberts
An animation rendered from the output of one simulation of Listeria monocytogenes motility. Microscale hops, as opposed to the nanoscale steps we investigate are apparent at this scale view. The bacterium induces an actin tail of variable density and demonstrates persistent motion.